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Mar 24, 2014
03/14
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Song, Yali; Yu, Jingjin; Huang, Bingru
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This article is from PLoS ONE , volume 9 . Abstract Elevated CO2 concentration may promote plant growth while high temperature is inhibitory for C3 plant species. The interactive effects of elevated CO2 and high temperatures on C3 perennial grass growth and carbon metabolism are not well documented. Kentucky bluegrass (Poa pratensis) plants were exposed to two CO2 levels (400 and 800 μmol mol−1) and five temperatures (15/12, 20/17, 25/22, 30/27, 35/32°C, day/night) in growth chambers....
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963838
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Feb 12, 2014
02/14
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Rathschlag, Marco; Memmert, Daniel
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This article is from Brain and Behavior , volume 4 . Abstract Background: The main aim of this pilot study was to investigate an advanced version of eye movement desensitization and reprocessing (EMDR) for reducing anxiety. Methods: Fifty participants were asked at two times of measurement (T1 and T2 with a rest of 4 weeks) to generate anxiety via the recall of autobiographical memories according to their anxiety. Furthermore, the participants were randomly assigned to an experimental group and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055185
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Sep 9, 2014
09/14
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Liu, Tao; Luo, Shenglin; Wang, Yang; Tan, Xu; Qi, Qingrong; Shi, Chunmeng
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This article is from Drug Design, Development and Therapy , volume 8 . Abstract Near-infrared (NIR) fluorescent sensors have emerged as promising molecular tools for cancer imaging and detection in living systems. However, cancer NIR fluorescent sensors are very challenging to develop because they are required to exhibit good specificity and low toxicity as an eligible contrast agent. Here, we describe the synthesis of a new heptamethine indocyanine dye (NIR-27) modified with a glycine at the...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166911
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2011
2011
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Vahedi, Shahram; Farrokhi, Farahman; Bevrani, Hossein
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This article is from Iranian Journal of Psychiatry , volume 6 . Abstract Objective: The aim of this study is to explore the confirmatory factor analysis results of the Persian adaptation of Statistics Anxiety Measure (SAM), proposed by Earp. Method: The validity and reliability assessments of the scale were performed on 298 college students chosen randomly from Tabriz University in Iran. Confirmatory factor analysis (CFA) was carried out to determine the factor structures of the Persian...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395949
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Jul 8, 2014
07/14
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Alves, Joao Guilherme B; de Araujo, Carla Adriane Fonseca Leal; Pontes, Isabelle E A; Guimaraes, Angelica C; Ray, Joel G
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This article is from BMC Pregnancy and Childbirth , volume 14 . Abstract Background: Preterm birth is the leading cause of infant mortality globally, including Brazil. We will evaluate whether oral magnesium citrate reduces the risk of placental dysfunction and its negative consequences for both the fetus and mother, which, in turn, should reduce the need for indicated preterm delivery. Methods/Design: We will complete a multicenter, randomized double-blind clinical trial comparing oral...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096428
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May 31, 2012
05/12
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Khodabandehloo, M; Shahrabadi, M Shamsi; Keyvani, H; Bambai, B; Sadigh, ZA
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This article is from Iranian Journal of Public Health , volume 41 . Abstract Background:: Rotaviruses cause diarrhea in infants and young children worldwide. Rotavirus outer capsid protein, VP7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. Many efforts have been done to produce recombinant VP7 that maintain native characteristics. We used baculovirus expression system to produce rotavirus VP7 protein and to study its immunogenicity....
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468985
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Apr 7, 2014
04/14
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Shanmugam, Raghuvaran; Aklujkar, Muktak; Schafer, Matthias; Reinhardt, Richard; Nickel, Olaf; Reuter, Gunter; Lovley, Derek R.; Ehrenhofer-Murray, Ann; Nellen, Wolfgang; Ankri, Serge; Helm, Mark; Jurkowski, Tomasz P.; Jeltsch, Albert
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This article is from Nucleic Acids Research , volume 42 . Abstract Dnmt2 enzymes are conserved in eukaryotes, where they methylate C38 of tRNA-Asp with high activity. Here, the activity of one of the very few prokaryotic Dnmt2 homologs from Geobacter species (GsDnmt2) was investigated. GsDnmt2 was observed to methylate tRNA-Asp from flies and mice. Unexpectedly, it had only a weak activity toward its matching Geobacter tRNA-Asp, but methylated Geobacter tRNA-Glu with good activity. In agreement...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041430
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Apr 1, 2014
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Mohamed, Saleh A; Almulaiky, Yaaser Q; Ahmed, Youssri M; Al-Bar, Omar AM; Ibrahim, Ibrahim H
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This article is from BMC Complementary and Alternative Medicine , volume 14 . Abstract Background: The miswak (Salvadora persica) is a natural toothbrush. It is well known that very little information has been reported on enzymes in miswak as medicinal plant. Recently, we study peroxidase in miswak. In the present study, the main goal of this work is to purify and characterize α-amylase from miswak. The second goal is to study the storage stability of α-amylase in toothpaste. Method: The...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974154
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Jun 24, 2011
06/11
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Barresi, Rita
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This article is from Skeletal Muscle , volume 1 . Abstract Muscular dystrophies are a large heterogeneous group of inherited diseases that cause progressive muscle weakness and permanent muscle damage. Very few muscular dystrophies show sufficient specific clinical features to allow a definite diagnosis. Because of the currently limited capacity to screen for numerous genes simultaneously, muscle biopsy is a time and cost-effective test for many of these disorders. Protein analysis interpreted...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156647
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Apr 17, 2014
04/14
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Fu, Weihua; Zhou, Zhansong; Liu, Shijian; Li, Qianwei; Yao, Jiwei; Li, Weibing; Yan, Junan
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This article is from PLoS ONE , volume 9 . Abstract Background: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is one of the risk factors of impaired male fertility potential. Studies have investigated the effect of CP/CPPS on several semen parameters but have shown inconsistent results. Hence, we performed a systematic literature review and meta-analysis to assess the association between CP/CPPS and basic semen parameters in adult men. Methods: Systematic literature searches were...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990624
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Feb 21, 2014
02/14
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Castillo, Jorge J; Iyengar, Meera; Kuritzky, Benjamin; Bishop, Kenneth D
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This article is from OncoTargets and therapy , volume 7 . Abstract In the last decade, the advent of biological targeted therapies has revolutionized the management of several types of cancer, especially in the realm of hematologic malignancies. One of these pathways, and the center of this review, is the phosphatidylinositol-3-kinase (PI3K) pathway. The PI3K pathway seems to play an important role in the pathogenesis and survival advantage in hematologic malignancies, such as leukemia,...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937185
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Dec 6, 2012
12/12
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Rossi, Settimio; Testa, Francesco; Li, Anren; Yaylacioglu, Fulya; Gesualdo, Carlo; Hejtmancik, J Fielding; Simonelli, Francesca
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This article is from The British Journal of Ophthalmology , volume 97 . Abstract Aim: The aim of the study was to describe the clinical and genetic features of 15 Italian patients with Bietti crystalline dystrophy (BCD). Methods: All study participants underwent a complete ophthalmological examination, including standard electroretinogram (ERG), optical coherence tomography, microperimetry, autofluorescence and multifocal electroretinogram. The 11 exons of the CYP4V2 gene were sequenced. The...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582089
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Dec 20, 2013
12/13
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Craparo, Giuseppe; Schimmenti, Adriano; Caretti, Vincenzo
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This article is from European Journal of Psychotraumatology , volume 4 . Abstract Background: The link between early traumatic experiences of abuse/neglect and criminal behaviour has been widely demonstrated. Less is known, however, about the relationship between these experiences and the development of psychopathic personality. Objective: This study investigated childhood relational trauma in a group of violent offenders from Italy. We hypothesised a higher level of early relational trauma...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871837
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Jun 21, 2012
06/12
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Zakar, Rubeena; Zakar, Muhammad Z; Faist, Thomas; Kraemer, Alexander
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This article is from SpringerPlus , volume 1 . Abstract This paper addresses the issue of intimate partner violence against women and its related immigration stressors in Pakistani immigrant families in Germany. Drawing on 32 in-depth interviews with Pakistani women in three cities in Germany, we found that psychological violence was the commonly reported violence among the study participants. The data showed that the process of immigration exacerbated tensions between spouses because of...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581110
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2011
2011
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Malakouti, Seyed Kazem; Nojomi, Marzieh; Salehi, Maryam; Bijari, Bita
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This article is from Iranian Journal of Psychiatry , volume 6 . Abstract Objective: In the last 30 years, ever since the PHC network in Iran has been established, the job duties of Behvarzes (Mental Health Workers in rural areas) have been constantly increasing. The aim of this study was to determine the burnout level, mental health status and the severity of occupational stress among Behvarzes. Methods: All Behvarzes (227) working in areas covered by Iran University of Medical Sciences were...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395939
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Nov 8, 2011
11/11
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Yook, Karen; Harris, Todd W.; Bieri, Tamberlyn; Cabunoc, Abigail; Chan, Juancarlos; Chen, Wen J.; Davis, Paul; de la Cruz, Norie; Duong, Adrian; Fang, Ruihua; Ganesan, Uma; Grove, Christian; Howe, Kevin; Kadam, Snehalata; Kishore, Ranjana; Lee, Raymond; Li, Yuling; Muller, Hans-Michael; Nakamura, Cecilia; Nash, Bill; Ozersky, Philip; Paulini, Michael; Raciti, Daniela; Rangarajan, Arun; Schindelman, Gary; Shi, Xiaoqi; Schwarz, Erich M.; Ann Tuli, Mary; Van Auken, Kimberly; Wang, Daniel; Wang,...
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This article is from Nucleic Acids Research , volume 40 . Abstract Since its release in 2000, WormBase (http://www.wormbase.org) has grown from a small resource focusing on a single species and serving a dedicated research community, to one now spanning 15 species essential to the broader biomedical and agricultural research fields. To enhance the rate of curation, we have automated the identification of key data in the scientific literature and use similar methodology for data extraction. To...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245152
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163
Aug 25, 2014
08/14
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Maletic, Vladimir; Raison, Charles
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This article is from Frontiers in Psychiatry , volume 5 . Abstract From a neurobiological perspective there is no such thing as bipolar disorder. Rather, it is almost certainly the case that many somewhat similar, but subtly different, pathological conditions produce a disease state that we currently diagnose as bipolarity. This heterogeneity – reflected in the lack of synergy between our current diagnostic schema and our rapidly advancing scientific understanding of the condition – limits...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142322
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Mar 20, 2013
03/13
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Marczyk, Michal; Jaksik, Roman; Polanski, Andrzej; Polanska, Joanna
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This article is from BMC Bioinformatics , volume 14 . Abstract Background: DNA microarrays are used for discovery of genes expressed differentially between various biological conditions. In microarray experiments the number of analyzed samples is often much lower than the number of genes (probe sets) which leads to many false discoveries. Multiple testing correction methods control the number of false discoveries but decrease the sensitivity of discovering differentially expressed genes....
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637832
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Apr 1, 2014
04/14
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Racis, Loretta; Storti, Eugenia; Pugliatti, Maura; Agnetti, Virgilio; Tessa, Alessandra; Santorelli, Filippo M
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This article is from BMC Medical Genetics , volume 15 . Abstract Background: The hereditary spastic paraplegias (HSPs) are pleiomorphic disorders of motor pathway and a large number of affected genes have been discovered. Yet, mutations in SPG4/SPAST represent the most frequent molecular etiology in autosomal dominant (AD) patients and sporadic cases. We describe a large, AD-HSP Sardinian family where 5 out of several living members harbored a novel deletion affecting also the 5′UTR of SPAST...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974227
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995
Feb 28, 2013
02/13
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Amiri, Mohammad Sadegh; Joharchi, Mohammad Reza
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This article is from Avicenna Journal of Phytomedicine , volume 3 . Abstract Objective: An ethnobotanical survey on the medicinal plant species marketed in Mashhad city, northeastern Iran, was conducted in order to document traditional medicinal knowledge and application of medicinal plants. Materials and Methods: This study was undertaken between 2011 and 2012. The indigenous knowledge of traditional healers used for medicinal purposes were collected through questionnaire and personal...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075713
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90
May 29, 2014
05/14
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Zhang, L; Zhang, T; Wang, L; Shao, S; Chen, Z; Zhang, Z
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This article is from Gene Therapy , volume 21 . Abstract Short hairpin RNA (shRNA)-mediated gene regulation is a commonly used technique for gene manipulation. An efficient and safe delivery system is indispensable when shRNA is delivered into living organisms for gene therapy. Previous studies have proved that DNA and protein can be delivered into dendritic cells (DCs) by non-pathogenic Saccharomyces cerevisiae without being degraded. CD40 is closely related to apoptosis of tumor cells and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086734
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73
Apr 17, 2014
04/14
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Yang, Qi; Xie, Yan-Dong; Zhang, Ming-xin; Huang, Bo; Zhang, Chao; Li, Hui-Yan; Zhang, Rong; Qin, Ming; Huang, Yu-Xin; Wang, Jing-Jie
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This article is from BMC Complementary and Alternative Medicine , volume 14 . Abstract Background: Electroacupuncture (EA) stimulation has been shown to have a great therapeutic potential for treating gastrointestinal motility disorders. However, no evidence has clarified the mechanisms contributing to the effects of EA stimulation at the Zusanli acupoint (ST.36). This study was designed to investigate the regulative effect of EA stimulation at the ST.36 on gastric motility and to explore its...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021071
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55
Nov 12, 2013
11/13
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Wright, Jill; Schneider, Brandt L
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This article is from Molecular Systems Biology , volume 9 . Abstract None
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039377
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Feb 20, 2014
02/14
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Kourkouta, Lambrini; Papathanasiou, Ioanna V.
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This article is from Materia Socio-Medica , volume 26 . Abstract Good communication between nurses and patients is essential for the successful outcome of individualized nursing care of each patient. To achieve this, however, nurses must understand and help their patients, demonstrating courtesy, kindness and sincerity. Also they should devote time to the patient to communicate with the necessary confidentiality, and must not forget that this communication includes persons who surround the sick...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990376
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Sep 26, 2011
09/11
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Lim, Sun Young; Ha, Hee Sung; Kwon, Hyuk-Sang; Lee, Jin-Hee; Yim, Hyeon Woo; Yoon, Kun-Ho; Lee, Won-Chul; Son, Ho-Young; Park, Yong-Moon
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This article is from Epidemiology and Health , volume 33 . Abstract OBJECTIVES: We aimed to determine the characteristics affecting insulin resistance in non-obese middle-aged adults in a rural community. METHODS: A total of 1,270 non-diabetic adults aged between 40 and 64 years old with body mass index (BMI) less than 25 kg/m2 were analyzed. Subjects with insulin resistance were defined as those who had the highest quartile value of the homeostasis model assessment of insulin resistance...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195816
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159
Jul 23, 2012
07/12
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Lakhan, Shaheen E; Kirchgessner, Annette
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This article is from Brain and Behavior , volume 2 . Abstract Prescription stimulants are often used to treat attention deficit hyperactivity disorder (ADHD). Drugs like methylphenidate (Ritalin, Concerta), dextroamphetamine (Dexedrine), and dextroamphetamine-amphetamine (Adderall) help people with ADHD feel more focused. However, misuse of stimulants by ADHD and nonaffected individuals has dramatically increased over recent years based on students' misconceptions or simple lack of knowledge of...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489818
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Feb 24, 2014
02/14
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Yeleswarapu, SriJyothsna; Rao, Aditya; Joseph, Thomas; Saipradeep, Vangala Govindakrishnan; Srinivasan, Rajgopal
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This article is from BMC Medical Informatics and Decision Making , volume 14 . Abstract Background: Pharmacovigilance aims to uncover and understand harmful side-effects of drugs, termed adverse events (AEs). Although the current process of pharmacovigilance is very systematic, the increasing amount of information available in specialized health-related websites as well as the exponential growth in medical literature presents a unique opportunity to supplement traditional adverse event...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936866
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55
Dec 9, 2013
12/13
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POJE, G.; ZINREICH, J.S.; SKITARELIC, N.; ?URIC VUKOVIC, K.; PASSALI, G.C.; PASSALI, D.; MLADINA, R.
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This article is from Acta Otorhinolaryngologica Italica , volume 34 . Abstract Septal deformities are very frequent in patients suffering from chronic rhinosinusitis (CRS). The question is whether or not some types of septal deformities are involved more frequently in this process or not. The authors observed the incidence of particular types of septal deformities in a group of CRS patients using Mladina classification. The same has been done with a control group that consisted of healthy...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025178
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153
Aug 5, 2014
08/14
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Hanson, Corrine; Rutten, Erica P; Wouters, Emiel FM; Rennard, Stephen
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This article is from International Journal of Chronic Obstructive Pulmonary Disease , volume 9 . Abstract The global increase in the prevalence and incidence of obesity has called serious attention to this issue as a major public health concern. Obesity is associated with many chronic diseases, including cardiovascular disease and diabetes, and recently the role of overweight and obesity in lung disease has received new interest. Independently of obesity, diet also plays a role as a risk factor...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4130708
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100
Feb 24, 2012
02/12
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Petrov, Pavel A.; Naumov, Dmitry Yu.; Konchenko, Sergey N.
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This article is from Acta Crystallographica Section E: Structure Reports Online , volume 68 . Abstract The octahedral cluster core of the anion in the structure of the title compound, (C16H36N)3[Mo6(C4H9S)6(μ3-Cl)7(μ3-S)]·2H2O, has -3 site symmetry. Two μ3-Cl atoms fully occupy positions in the cluster core, while the remaining six positions are statistically occupied by Cl and S atoms in a 1:5 ratio. The fully occupied Cl-atom positions are located on sites with 3 symmetry, and the N...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297277
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204
Dec 16, 2013
12/13
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Seyfried, Thomas N.; Flores, Roberto E.; Poff, Angela M.; D'Agostino, Dominic P.
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This article is from Carcinogenesis , volume 35 . Abstract Emerging evidence indicates that cancer is primarily a metabolic disease involving disturbances in energy production through respiration and fermentation. The genomic instability observed in tumor cells and all other recognized hallmarks of cancer are considered downstream epiphenomena of the initial disturbance of cellular energy metabolism. The disturbances in tumor cell energy metabolism can be linked to abnormalities in the...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941741
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Feb 13, 2013
02/13
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Kislukhin, Victor V
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This article is from Theoretical Biology & Medical Modelling , volume 10 . Abstract The aim. (1) To develop a mathematical model of the passage of a diffusible indicator through microcirculation based on a stochastic description of diffusion and flow; (2) To use Goresky transform of the dilution curves of the diffusible indicators for the estimation of the permeability of a tissue-capillary barrier.The method. We assume that there are two causes for flow to be stochastic: (a) All...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584909
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Sep 4, 2013
09/13
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Diene, Ousmane; Wang, Wei; Narisawa, Kazuhiko
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This article is from Microbes and Environments , volume 28 . Abstract A dark septate fungus of Pseudosigmoidea, Hyphomycetes, was recovered from forest soil in Ibaraki prefecture, Japan. The isolate is characterized by pale to brown conidia with up to 8 septa measuring 68–132 × 4–7.9 μm. It is also unique in producing conidia borne by long conidogenious cells in agar medium with or without water, compared to P. cranei, which must be immersed in water to sporulate. Morphological analysis...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070967
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Mar 22, 2012
03/12
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Zhang, Zhang; Li, Jun; Cui, Peng; Ding, Feng; Li, Ang; Townsend, Jeffrey P; Yu, Jun
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This article is from BMC Bioinformatics , volume 13 . Abstract Background: Genetic mutation, selective pressure for translational efficiency and accuracy, level of gene expression, and protein function through natural selection are all believed to lead to codon usage bias (CUB). Therefore, informative measurement of CUB is of fundamental importance to making inferences regarding gene function and genome evolution. However, extant measures of CUB have not fully accounted for the quantitative...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368730
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Apr 19, 2012
04/12
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Seaton, Sarah E; King, Sophie; Manktelow, Bradley N; Draper, Elizabeth S; Field, David J
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This article is from Archives of Disease in Childhood. Fetal and Neonatal Edition , volume 98 . Abstract Objective: To assess the care given to the babies born at the threshold of viability over the last 20 years using regional and national data. Design: Population-based retrospective study. Setting: Former ‘Trent’ health region. Participants: Babies born between 1 January 1991 and 31 December 2010 at 22+0 to 25+6 weeks gestational age. Main outcome measure: Survival and use of respiratory...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479086
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Oct 3, 2012
10/12
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Sereno, Paul C.
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This article is from ZooKeys . Abstract AbstractHeterodontosaurids comprise an important early radiation of small-bodied herbivores that persisted for approximately 100 My from Late Triassic to Early Cretaceous time. Review of available fossils unequivocally establishes Echinodon as a very small-bodied, late-surviving northern heterodontosaurid similar to the other northern genera Fruitadens and Tianyulong. Tianyulong from northern China has unusual skeletal proportions, including a relatively...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491919
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Aug 21, 2012
08/12
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Diazgranados, Mauricio
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This article is from PhytoKeys . Abstract AbstractThe páramos and high Andean forests of the tropical Andes are largely dominated by frailejones (Nomen nudum Cuatrec., Nomen nudum). These plants are ecologically and culturally essential for both ecosystems and local inhabitants. The frailejones have been studied for over two centuries, but the taxonomic knowledge is still sparse and incomplete. The inedited monograph by Cuatrecasas contains only ca. 70% of the species known today, and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492930
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May 24, 2013
05/13
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Koenderink, Jan; Wijntjes, Maarten; van Doorn, Andrea
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This article is from i-Perception , volume 4 . Abstract The “zograscope” is a “visual aid” (commonly known as “optical machine” in the 18th century) invented in the mid-18th century, and in general use until the early 20th century. It was intended to view single pictures (thus not stereographic pairs) with both eyes. The optics approximately eliminates the physiological cues (binocular disparity, vergence, accommodation, movement parallax, and image blur) that might indicate the...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690410
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Aug 9, 2013
08/13
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Saifan, Chadi; Saad, Marc; El-Charabaty, Elie; El-Sayegh, Suzanne
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This article is from International Journal of General Medicine , volume 6 . Abstract Calciphylaxis is a challenging complication of end-stage renal disease, with an unknown underlying mechanism. Several risk factors have been identified, such as hyperphosphatemia, hypercalcemia, hyperparathyroidism, low serum albumin levels, and history of warfarin therapy. This article presents a case of calciphylaxis provoked by reintroduction of warfarin therapy, introducing the possibility of direct...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745288
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Jul 3, 2014
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Sudheimer, Keith D.; O'Hara, Ruth; Spiegel, David; Powers, Bevin; Kraemer, Helena C.; Neri, Eric; Weiner, Michael; Hardan, Antonio; Hallmayer, Joachim; Dhabhar, Firdaus S.
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This article is from Frontiers in Aging Neuroscience , volume 6 . Abstract Separate bodies of literature report that elevated pro-inflammatory cytokines and cortisol negatively affect hippocampal structure and cognitive functioning, particularly in older adults. Although interactions between cytokines and cortisol occur through a variety of known mechanisms, few studies consider how their interactions affect brain structure. In this preliminary study, we assess the impact of interactions...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079951
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Jul 8, 2014
07/14
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Liu, Ying; Zhang, Wei; Wang, Xiao-Jie; Liu, Shi
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This article is from BMC Complementary and Alternative Medicine , volume 14 . Abstract Background: Kanglaite® injection (KLT), with a main ingredient of Coix seed oil (a traditional Chinese medicine), has been widely used for cancer treatment in China. KLT has an inhibitory effect on many kinds of tumors and PI3K/Akt/mTOR signaling promotes cell survival, proliferation, and progression in cancer cells. Therefore, targeting this pathway may lead to the development of novel therapeutic...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105135
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Jun 21, 2014
06/14
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Petasch, Jan; Disch, Eva-Maria; Markert, Stephanie; Becher, Dorte; Schweder, Thomas; Huttel, Bruno; Reinhardt, Richard; Harder, Jens
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eye 61
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This article is from BMC Microbiology , volume 14 . Abstract Background: The facultatively anaerobic betaproteobacterium Castellaniella defragrans 65Phen utilizes acyclic, monocyclic and bicyclic monoterpenes as sole carbon source under oxic as well as anoxic conditions. A biotransformation pathway of the acyclic β-myrcene required linalool dehydratase-isomerase as initial enzyme acting on the hydrocarbon. An in-frame deletion mutant did not use myrcene, but was able to grow on monocyclic...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109377
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Aug 21, 2014
08/14
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Koh, Young Rae; Kim, Shine Young; Kim, In Suk; Chang, Chulhun L.; Lee, Eun Yup; Son, Han Chul; Kim, Hyung Hoi
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This article is from Annals of Laboratory Medicine , volume 34 . Abstract We performed customer satisfaction surveys for physicians and nurses regarding clinical laboratory services, and for outpatients who used phlebotomy services at a tertiary care unit level to evaluate our clinical laboratory and phlebotomy services. Thus, we wish to share our experiences with the customer satisfaction survey for clinical laboratory and phlebotomy services. Board members of our laboratory designed a study...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151008
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Jun 6, 2012
06/12
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Bonner, Jennifer; Letko, Michael; Nikolaus, Oliver Brant; Krug, Lisa; Cooper, Alexandria; Chadwick, Benjamin; Conklin, Phoebe; Lim, Amy; Chien, Chi-Bin; Dorsky, Richard I
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This article is from Neural Development , volume 7 . Abstract Background: Growth cone navigation across the vertebrate midline is critical in the establishment of nervous system connectivity. While midline crossing is achieved through coordinated signaling of attractive and repulsive cues, this has never been demonstrated at the single cell level. Further, though growth cone responsiveness to guidance cues changes after crossing the midline, it is unclear whether midline crossing itself is...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507651
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May 29, 2012
05/12
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Ishaque, Sana; Shamseer, Larissa; Bukutu, Cecilia; Vohra, Sunita
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This article is from BMC Complementary and Alternative Medicine , volume 12 . Abstract Background: Rhodiola rosea (R. rosea) is grown at high altitudes and northern latitudes. Due to its purported adaptogenic properties, it has been studied for its performance-enhancing capabilities in healthy populations and its therapeutic properties in a number of clinical populations. To systematically review evidence of efficacy and safety of R. rosea for physical and mental fatigue. Methods: Six...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541197
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Jan 4, 2013
01/13
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Xu, Junjie; An, Minghui; Han, Xiaoxu; Jia, Manhong; Ma, Yanling; Zhang, Min; Hu, Qinghai; Chu, Zhenxing; Zhang, Jing; Jiang, Yongjun; Geng, Wenqing; Lu, Lin; Shang, Hong
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This article is from BMC Infectious Diseases , volume 13 . Abstract Background: Yunnan has the largest number of reported HIV/AIDS cases among all Chinese provinces, the reported prevalence of HIV among Yunnan men who have sex with men (MSM) passed 10%, while HIV incidence epidemic and molecular characteristics of new infected Yunnan MSM were not evaluated before. Methods: An 18 months prospective followed up with a frequency of 3 month per visit were conducted among HIV seronegative MSM in...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599414
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Oct 5, 2011
10/11
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Salami, MA; Adeoye, PO; Adegboye, VO; Adebo, OA
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This article is from Cardiovascular Journal of Africa , volume 23 . Abstract Background: Effusive–constrictive pericarditis is a syndrome in which constriction by the visceral pericardium occurs in the presence of a dense effusion in a free pericardial space. Treatment of this disease is problematic because pericardiocentesis does not relieve the impaired filling of the heart and surgical removal of the visceral pericardium is challenging. We sought to provide further information by...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721937
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Oct 20, 2013
10/13
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Kang, Mi-Kyung; Nam, Bo-Ra; Lee, Yun-Seob; Cheon, Song-Hee
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This article is from Journal of Physical Therapy Science , volume 25 . Abstract [Purpose] The purpose of the present study was to investigate the effect of TENS applied to the skin overlying the bellies of the gastrocnemius muscles of the lower limbs on balance and plantar pressure of healthy adults. [Subjects and Methods] Twenty-eight healthy college students were the subjects of this study. Adhesive transcutaneous electrical nerve stimulation (TENS) electrodes were attached to the medial and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818762
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Oct 16, 2012
10/12
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Bachir Benjelloun, EI; Ahallal, Youness; Khatala, Khalid; Souiki, Tarik; Kamaoui, Iman; Taleb, Khalid Ati
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This article is from Urology Annals , volume 5 . Abstract Context:: Impalement injuries of the rectum with bladder perforation have been rarely reported. Such lesions have been associated with increased postoperative morbidity. A well-conducted preoperative evaluation of the lesions tends to prevent such complications. Aims:: To increase awareness about patients with rectal impalement that involve bladder injuries and to examine the significance of thorough clinical examination and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835982
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Jan 17, 2014
01/14
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Song, Fei; Poljak, Anne; Kochan, Nicole A; Raftery, Mark; Brodaty, Henry; Smythe, George A; Sachdev, Perminder S
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This article is from Proteome Science , volume 12 . Abstract Background: With the promise of disease modifying treatments, there is a need for more specific diagnosis and prognosis of Alzheimer’s disease (AD) and mild cognitive impairment (MCI). Plasma biomarkers are likely to be utilised to increase diagnostic accuracy and specificity of AD and cognitive decline. Methods: Isobaric tags (iTRAQ) and proteomic methods were used to identify potential plasma biomarkers of MCI and AD. Relative...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898732
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Mar 19, 2014
03/14
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Grimaldi-Bensouda, Lamiae; Begaud, Bernard; Rossignol, Michel; Avouac, Bernard; Lert, France; Rouillon, Frederic; Benichou, Jacques; Massol, Jacques; Duru, Gerard; Magnier, Anne-Marie; Abenhaim, Lucien; Guillemot, Didier
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This article is from PLoS ONE , volume 9 . Abstract Background: Prescribing of antibiotics for upper respiratory tract infections (URTI) varies substantially in primary care. Objectives: To describe and compare antibiotic and antipyretic/anti-inflammatory drugs use, URTI symptoms' resolution and occurrence of potentially-associated infections in patients seeking care from general practitioners (GPs) who exclusively prescribe conventional medications (GP-CM), regularly prescribe homeopathy...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960096
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Oct 20, 2013
10/13
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Barbas, Carmen Silvia Valente; Isola, Alexandre Marini; Farias, Augusto Manoel de Carvalho; Cavalcanti, Alexandre Biasi; Gama, Ana Maria Casati; Duarte, Antonio Carlos Magalhaes; Vianna, Arthur; Serpa, Ary; Bravim, Bruno de Arruda; Pinheiro, Bruno do Valle; Mazza, Bruno Franco; de Carvalho, Carlos Roberto Ribeiro; Toufen, Carlos; David, Cid Marcos Nascimento; Taniguchi, Corine; Mazza, Debora Dutra da Silveira; Dragosavac, Desanka; Toledo, Diogo Oliveira; Costa, Eduardo Leite; Caser, Eliana...
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This article is from Revista Brasileira de Terapia Intensiva , volume 26 . Abstract Perspectives on invasive and noninvasive ventilatory support for critically ill patients are evolving, as much evidence indicates that ventilation may have positive effects on patient survival and the quality of the care provided in intensive care units in Brazil. For those reasons, the Brazilian Association of Intensive Care Medicine (Associação de Medicina Intensiva Brasileira - AMIB) and the Brazilian...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103936
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Jul 24, 2014
07/14
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Parikh, Ankur P.; Curtis, Ross E.; Kuhn, Irene; Becker-Weimann, Sabine; Bissell, Mina; Xing, Eric P.; Wu, Wei
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This article is from PLoS Computational Biology , volume 10 . Abstract The HMT3522 progression series of human breast cells have been used to discover how tissue architecture, microenvironment and signaling molecules affect breast cell growth and behaviors. However, much remains to be elucidated about malignant and phenotypic reversion behaviors of the HMT3522-T4-2 cells of this series. We employed a “pan-cell-state” strategy, and analyzed jointly microarray profiles obtained from different...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109850
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Aug 14, 2014
08/14
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Gomez-Jimenez, Silvia; Noriega-Orozco, Lorena; Sotelo-Mundo, Rogerio R.; Cantu-Robles, Vito A.; Cobian-Guemes, Ana G.; Cota-Verdugo, Rosario G.; Gamez-Alejo, Luis A.; del Pozo-Yauner, Luis; Guevara-Hernandez, Eduardo; Garcia-Orozco, Karina D.; Lopez-Zavala, Alonso A.; Ochoa-Leyva, Adrian
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This article is from Genome Announcements , volume 2 . Abstract The high-quality draft genomes of two Vibrio parahaemolyticus strains, one that causes the acute hepatopancreatic necrosis disease (AHPND) in cultured shrimps (FIM-S1708+), and another that does not (FIM-S1392−) are reported. A chromosome-scale assembly for the FIM-S1392− genome is reported here. The analysis of the two genomes gives some clues regarding the genomic differences between the strains.
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132621
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Mar 26, 2013
03/13
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Hardy, Timothy; Wells, Christopher; Kendrick, Stuart; Hudson, Mark; Day, Christopher P; Burt, Alastair D; Masson, Steven; Stewart, Stephen F
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This article is from BMC Gastroenterology , volume 13 . Abstract Background: Patients with suspected alcoholic hepatitis and a Discriminant Function ≥32 underwent liver biopsy to confirm the diagnosis. Of these (n = 58), 43 had histological features of alcoholic hepatitis and 15 (25%) did not.We aimed to determine the laboratory features that differentiated those patients with a histological diagnosis of alcoholic hepatitis from those without, and assess potential clinical utility....
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3631132
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May 8, 2013
05/13
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Zhong, Nanjing; Gui, Zhongyu; Xu, Li; Huang, Jianrong; Hu, Kun; Gao, Yongqing; Zhang, Xia; Xu, Zhenbo; Su, Jianyu; Li, Bing
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This article is from Lipids in Health and Disease , volume 12 . Abstract Background: Pure 1, 3-diacylglycerols (1, 3-DAG) have been considered to be significant surfactants in food, cosmetics and pharmaceutical industries, as well as the effect on obesity prevention. Methods: In this study, a vacuum-driven air bubbling operation mode was developed and evaluated for the enzymatic synthesis of 1, 3-DAG of saturated fatty acids, by direct esterification of glycerol with fatty acids in a...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680111
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Aug 16, 2013
08/13
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Aspinall, Richard; Govind, Sheila; Lapenna, Antonio; Lang, Pierre Olivier
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This article is from Immunity & Ageing : I & A , volume 10 . Abstract Transfusion of autologous leukocytes after prolonged storage has been proposed as a means of rejuvenating the immune system of older individuals. The rationale for this approach is that age related immune decline is associated with a diminished pool of naïve T cells following atrophy of the thymus and reduction in thymic output. The presence of high levels of naïve T cells within the blood of young individuals could...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765176
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Jul 3, 2014
07/14
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Lee, Jung-Yun
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This article is from Journal of Gynecologic Oncology , volume 25 . Abstract None
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102745
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Jun 1, 2014
06/14
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Donaduzzi, Liziane C.; De-Conto, Ferdinando; Kuze, Luana S.; Rovani, Gisele; Flores, Mateus E.; Pasqualotti, Adriano
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This article is from Journal of Clinical and Experimental Dentistry , volume 6 . Abstract Introduction: Squamous cell carcinoma represents about 90% of malignancies of the mouth and about 38% of the head and neck tumors. The behavior of the cancer is very aggressive, presenting early cervical metastasis and, often contralateral ranging from 0.9 to 36%. Objectives: This study aims to analyze clinical and pathological factors that may influence metastasis of squamous cell carcinoma in cervical...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134846
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Mar 10, 2008
03/08
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Veggeland, Turid; Dyb, Sigurd
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This article is from Pharmacy Practice , volume 6 . Abstract The aim of the study was to use a clinical pharmacist in order to improve the medication of patients in a geriatric hospital unit. The hospital had no experience of using a clinical pharmacist before.Methods: A clinical pharmacist participated in the therapeutic team for 27 days during a 4-year period. Drug-related problems were recorded prospectively and discussed before and at the ward round. The results of the physician’s...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147275
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Sep 12, 2014
09/14
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Jiang, Yanxialei; Choi, Won Hoon; Lee, Jung Hoon; Han, Dong Hoon; Kim, Ji Hyeon; Chung, Young-Shin; Kim, Se Hyun; Lee, Min Jae
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This article is from Scientific Reports , volume 4 . Abstract In the arginylation branch of the N-end rule pathway, unacetylated N-terminal destabilizing residues function as essential determinants of protein degradation signals (N-degron). Here, we show that a neurostimulant, para-chloroamphetamine (PCA), specifically inhibits the Arg/N-end rule pathway, delaying the degradation of its artificial and physiological substrates, including regulators of G protein signaling 4 (RGS4), in vitro and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161967
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Apr 23, 2011
04/11
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Paigel, Altemar S; Ribeiro Junior, Rogerio F; Fernandes, Aurelia A; Targueta, Gabriel P; Vassallo, Dalton V; Stefanon, Ivanita
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This article is from Reproductive Biology and Endocrinology : RB&E , volume 9 . Abstract Background: Ovarian sex hormones (OSHs) are implicated in cardiovascular function. It has been shown that OSHs play an important role in the long term regulation of cardiac sarcoplasmic reticulum (SR) function and contractility, although early effects of OSHs deprivation on myocardial contractility have not yet been determined. This study evaluated the early and late effects of OSHs deficiency on left...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107166
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Dec 19, 2011
12/11
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Delgado Hurtado, Juan J; Pineda, Marcela; Cazali, Iris; Mejia, Carlos
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This article is from Journal of the International AIDS Society , volume 14 . Abstract Background: The prevalence of HIV/AIDS in Guatemala among the general population is 0.79%, and 94% of transmission is directly related to sexual contact. Studies have been conducted on high- prevalence HIV-positive populations (men who have sex with men, commercial sex workers and prisoners). Heterosexual transmission has gained importance in the epidemic in Central America. To our knowledge, no study...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267676
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Jan 20, 2012
01/12
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Starc, Gregor; Strel, Janko
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This article is from BMC Public Health , volume 12 . Abstract Background: This study was constructed as a comparison group pre-test/post-test quasi-experiment to assess the effect of the implementation of the PE curriculum by specialist PE teachers on children's physical development and physical fitness. Methods: 146 classes from 66 Slovenian primary schools were assigned to quasi-test (71) and quasi-control (75) groups. Data from the SLOFIT database was used to compare the differences in...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305363
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Oct 25, 2012
10/12
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Nishida, Atsushi; Nagahama, Kiyotaka; Imaeda, Hirotsugu; Ogawa, Atsuhiro; Lau, Cindy W.; Kobayashi, Taku; Hisamatsu, Tadakazu; Preffer, Frederic I.; Mizoguchi, Emiko; Ikeuchi, Hiroki; Hibi, Toshifumi; Fukuda, Minoru; Andoh, Akira; Blumberg, Richard S.; Mizoguchi, Atsushi
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This article is from The Journal of Experimental Medicine , volume 209 . Abstract The colitis-associated glycome mediates CD4+ T cell expansion and contributes to the exacerbation of T cell–mediated intestinal inflammation.
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526363
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Mar 8, 2013
03/13
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Jonsson, Lina; Nasholm, Anna; Roepstorff, Lars; Egenvall, Agneta; Dalin, Goran; Philipsson, Jan
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This article is from Acta Veterinaria Scandinavica , volume 55 . Abstract Background: Soundness is important for welfare and utility of the riding horse. Musculoskeletal disorders are the most common causes of interruption in training and of culling. Despite great importance, heritability of a majority of health traits in horses has previously not been estimated. The objective was to perform genetic analyses of medical and orthopaedic health traits in young riding horses, including estimates of...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606462
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Feb 23, 2013
02/13
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Livi, Carolina B.; Hardman, Rulon L.; Christy, Barbara A.; Dodds, Sherry G.; Jones, Diane; Williams, Charnae; Strong, Randy; Bokov, Alex; Javors, Martin A.; Ikeno, Yuji; Hubbard, Gene; Hasty, Paul; Sharp, Zelton Dave
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This article is from Aging (Albany NY) , volume 5 . Abstract Chronic treatment of mice with an enterically released formulation of rapamycin (eRapa) extends median and maximum life span, partly by attenuating cancer. The mechanistic basis of this response is not known. To gain a better understanding of these in vivo effects, we used a defined preclinical model of neuroendocrine cancer, Rb1+/− mice. Previous results showed that diet restriction (DR) had minimal or no effect on the lifespan of...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616197
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Oct 16, 2013
10/13
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Ding, Yi S.; Chan, C. T.; Wang, R. P.
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This article is from Scientific Reports , volume 3 . Abstract Materials with negative permittivity and permeability can overcome the diffraction limit, thereby making the sub-wavelength imaging possible. In this study, we analyze the effects of gradient index on a half-infinite perfect lens. We assume that the sharp interface between the vacuum and the negative-index material is replaced by a smooth transition profile such that the index gradually changing from positive to negative....
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3797428
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Jun 11, 2013
06/13
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Bakloushinskaya, Irina; Romanenko, Svetlana A.; Serdukova, Natalia A.; Graphodatsky, Alexander S.; Lyapunova, Elena A.
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eye 55
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This article is from Comparative Cytogenetics , volume 7 . Abstract AbstractThe subterranean mole vole, Ellobius tancrei, with aspecific variability in autosomes (2n = 31–54) and unusual sex chromosomes (XX in males and females), represents an amazing model for studying the role of chromosome changes in speciation. New materials from the upper reaches of the Surkhob River in the Pamiro-Alay mountains resulted in the discovery of a new form with 2n = 30. The application of Zoo-FISH and...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833758
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Jul 20, 2011
07/11
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Volk, Alexander E.; Carter, Oliver; Fricke, Julia; Herkenrath, Peter; Poggenborg, Jorg; Borck, Guntram; Demant, Andre W.; Ivo, Roland; Eysel, Peer; Kubisch, Christian; Neugebauer, Antje
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This article is from Molecular Vision , volume 17 . Abstract Purpose: Clinical and molecular characterization of patients with horizontal gaze palsy with progressive scoliosis (HGPPS) to extend existing knowledge of the phenotype caused by mutations in the Roundabout homolog of Drosophila 3 (ROBO3) gene. Methods: Four patients (aged 6 months to 13 years), two of them siblings, with features of horizontal gaze palsy and their parents were examined clinically and by molecular testing of the ROBO3...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154129
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Sep 30, 2010
09/10
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Farzin, M.; Panahandeh, H.
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This article is from Journal of Dentistry (Tehran, Iran) , volume 7 . Abstract Objective:: The aim of this study was to evaluate the dimensional stability of casts made from an alginate impression material poured immediately and stored after specific periods. Materials and Methods:: The common alginate used in Iran (Super; Iralgin, Golchai Co., Tehran, Iran) was tested. A master model was mounted on a special device and used to obtain the impressions. These impressions were stored at 23°C...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184758
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Jan 29, 2013
01/13
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Kang, Eun Kyeong; Lim, Jung Sub; Lee, Jun Ah; Kim, Dong Ho
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eye 56
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This article is from Journal of Korean Medical Science , volume 28 . Abstract We aimed to compare the immune response induced by natural infection with 2009 pandemic influenza A/H1N1 (pH1N1) virus and by monovalent pH1N1 vaccination in children and adolescents. This cross-sectional clinical study was conducted at 3 hospitals in Korea from February to May 2010. A total of 266 healthy subjects aged from 6 months to 18 yr were tested for the presence of the antibody against pH1N1 using...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565140
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Oct 29, 2012
10/12
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Rahman, Rakhshanda Layeequr; Pruthi, Sandhya
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This article is from Cancers , volume 4 . Abstract Women who are at high risk of breast cancer can be offered chemoprevention. Chemoprevention strategies have expanded over the past decade and include selective receptor modulator inhibitors and aromatase inhibitors. Physicians are expected to provide individualized risk assessments to identify high risk women who may be eligible for chemoprevention. It is prudent that physicians utilize a shared decision approach when counseling high risk women...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712728
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Jun 3, 2013
06/13
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Tulin, Sarah; Aguiar, Derek; Istrail, Sorin; Smith, Joel
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This article is from EvoDevo , volume 4 . Abstract Background: The de novo assembly of transcriptomes from short shotgun sequences raises challenges due to random and non-random sequencing biases and inherent transcript complexity. We sought to define a pipeline for de novo transcriptome assembly to aid researchers working with emerging model systems where well annotated genome assemblies are not available as a reference. To detail this experimental and computational method, we used early...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748831
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May 20, 2013
05/13
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Cerff, Martin; Scholz, Alexander; Franzreb, Matthias; Batalha, Iris L; Roque, Ana Cecilia A; Posten, Clemens
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This article is from BMC Biotechnology , volume 13 . Abstract Background: In situ magnetic separation (ISMS) has emerged as a powerful tool to overcome process constraints such as product degradation or inhibition of target production. In the present work, an integrated ISMS process was established for the production of his-tagged single chain fragment variable (scFv) D1.3 antibodies (“D1.3”) produced by E. coli in complex media. This study investigates the impact of ISMS on the overall...
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750846
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